The University of Kansas Molecular Probes Core (KU-MPC) offers a wide range of services to investigators interested in chemical biology. A major focus is to assist investigators that wish to create and study fluorescent molecules in biological systems. We offer custom synthesis of fluorescent small molecules, peptides, and other molecular probes as well as imaging of these agents in optically transparent zebrafish (in vivo). Studies of small molecules in zebrafish can be used to bridge the gap between assays in cultured cells and lower-resolution / higher cost experiments in rodents.  

Fluorescence and brightfield imaging of Danio rerio (zebrafish)

Prof. Blake Peterson, the Leader of the KU-MPC, has a diverse scientific background working at the interface between chemistry and biology.  In collaboration with his research laboratory in the KU Dept. of Medicinal Chemistry, this core facility provides investigators access to a broad range of fluorescent compounds for use in biological assays.

Dr. Chamani Perera, the Director of the KU-MPC, is a talented organic/medicinal chemist and chemical biologist specializing in the synthesis and evaluation of molecular probes of biological systems. Staff of the KU-MPC work with investigators on probe design, synthesis, assay development, high content imaging and screening, data acquisition, and data analysis.

Phenotypic screening of Danio rerio (zebrafish) embryos

This core facility houses Danio rerio (zebrafish) for research in chemical biology and drug discovery. In contrast to more expensive rodent models, the optical transparency of these animals make them more amenable to a wide variety of fluorescence-based assays. The imaging equipment of the KU-MPC includes a Zeiss Axio Zoom.V16 stereomicroscope (11x-412x magnification) equipped with a Hamamatsu Orca Flash 4.0 CMOS camera and Sutter DG4 fast filter switching illumination system for ratiometric and other fluorescence-based assays. This system is combined with a sensitive and tunable microinjection platform that allows for delivery and imaging of virtually any fluorescent molecule in vivo. The KU-MPC further offers on-site housing of strains of adult zebrafish, mammalian cell culture services for drug screening and cancer biology studies, and cryopreservation of cell lines. By establishing robust breeding protocols, the KU-MPC can generate large numbers of zebrafish embryos for phenotypic screening and discovery toxicology projects.  Investigators are encouraged to contact us for free consultation.

CMADP Events
Special seminar by Dr. Kevin W. Plaxco
Professor of Chemistry & Biochemistry
UC Santa Barbara

Wednesday, April 19, 2017 at 4:00pm
School of Pharmacy, Room 3020

"Counting molecules, dodging blood cells: real-time molecular measurements directly in the living body"
The development of technology capable of continuously tracking the levels of drugs, metabolites, and biomarkers in situ in the body would revolutionize our understanding of health and our ability to detect and treat disease. It would, for example, provide clinicians with a real-time window into organ function and would enable therapies guided by patient-specific, real-time pharmacokinetics, opening a new dimension in personalized medicine. In response my group has pioneered the development of a “biology-inspired” electrochemical approach to monitoring specific molecules that supports real-time measurements of arbitrary molecular targets (irrespective of their chemical reactivity) directly in awake, fully ambulatory subjects.